rabbit polyclonal anti human pde4a antibody Search Results


fibers  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank fibers
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phospho pde4a  (Novus Biologicals)
90
Novus Biologicals phospho pde4a
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rabbit anti-pde4a antibody df 13317  (Affinity Biosciences)
90
Affinity Biosciences rabbit anti-pde4a antibody df 13317
Primers used in the Gene expression analysis.
Rabbit Anti Pde4a Antibody Df 13317, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal rabbit anti-pde4a igg  (FabGennix)
90
FabGennix polyclonal rabbit anti-pde4a igg
Primers used in the Gene expression analysis.
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pde4a  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc pde4a
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
Pde4a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti pde4a  (Proteintech)
92
Proteintech rabbit anti pde4a
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
Rabbit Anti Pde4a, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sc 8066 anti disc1 rabbit wb  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology sc 8066 anti disc1 rabbit wb
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
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pde4a  (Abcam)
97
Abcam pde4a
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
Pde4a, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti pde4a antibody  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology rabbit anti pde4a antibody
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
Rabbit Anti Pde4a Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-pde4d  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology rabbit anti-pde4d
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
Rabbit Anti Pde4d, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-rabbit pde4a polyclonal antibody  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology anti-rabbit pde4a polyclonal antibody
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
Anti Rabbit Pde4a Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom-made pde4b antibody  (GenScript corporation)
90
GenScript corporation custom-made pde4b antibody
a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of <t>PDE4A,</t> PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.
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Image Search Results


Primers used in the Gene expression analysis.

Journal: Brain Sciences

Article Title: Moderate-Intensity Intermittent Training Alters the DNA Methylation Pattern of PDE4D Gene in Hippocampus to Improve the Ability of Spatial Learning and Memory in Aging Rats Reduced by D-Galactose

doi: 10.3390/brainsci13030422

Figure Lengend Snippet: Primers used in the Gene expression analysis.

Article Snippet: The blots were blocked in 5% BSA in Tris-Buffered Saline with Tween (TBST) and incubated with rabbit anti-PDE4A antibody (DF 13317, 1:500, Affinity Biosciences Cincinnati, OH, USA), rabbit anti-PDE4B antibody (72096, 1:1000, CST), rabbit anti-PDE4D antibody (DF6535, 1:800, Affinity Biosciences Cincinnati, OH, USA), rabbit anti-PKA antibody (AF5450, 1:800, Affinity Biosciences Cincinnati, OH, USA), rabbit anti-CREB antibody (9197, 1:1000, CST), rabbit anti-p-CREB antibody (9198, 1:800, CST), rabbit anti-BDNF antibody (DF6387, 1:700, Affinity Biosciences Cincinnati, OH, USA), and rabbit anti-GAPDH antibody (5174, 1:1000, CST).

Techniques: Gene Expression

Effects of MIIT on the expression of PDE4A/B/D mRNA and protein in aging rats: ( a ) The expression of PDE4A/B/D mRNA in hippocampus was detected by Real-Time PCR (mean ± SEM, n = 6). ( b – e ) Western blot analysis of PDE4A/B/D protein expression levels (mean ± SEM, n = 3). Independent-Samples t Test, * p < 0.05 vs. D-Gal group, ** p < 0.01 vs. D-Gal group. Control: young group treated with saline, D-Gal Group: aging group induced by D-galactose, D-Gal+E Group: aging group induced by D-galactose that performed treadmill exercise.

Journal: Brain Sciences

Article Title: Moderate-Intensity Intermittent Training Alters the DNA Methylation Pattern of PDE4D Gene in Hippocampus to Improve the Ability of Spatial Learning and Memory in Aging Rats Reduced by D-Galactose

doi: 10.3390/brainsci13030422

Figure Lengend Snippet: Effects of MIIT on the expression of PDE4A/B/D mRNA and protein in aging rats: ( a ) The expression of PDE4A/B/D mRNA in hippocampus was detected by Real-Time PCR (mean ± SEM, n = 6). ( b – e ) Western blot analysis of PDE4A/B/D protein expression levels (mean ± SEM, n = 3). Independent-Samples t Test, * p < 0.05 vs. D-Gal group, ** p < 0.01 vs. D-Gal group. Control: young group treated with saline, D-Gal Group: aging group induced by D-galactose, D-Gal+E Group: aging group induced by D-galactose that performed treadmill exercise.

Article Snippet: The blots were blocked in 5% BSA in Tris-Buffered Saline with Tween (TBST) and incubated with rabbit anti-PDE4A antibody (DF 13317, 1:500, Affinity Biosciences Cincinnati, OH, USA), rabbit anti-PDE4B antibody (72096, 1:1000, CST), rabbit anti-PDE4D antibody (DF6535, 1:800, Affinity Biosciences Cincinnati, OH, USA), rabbit anti-PKA antibody (AF5450, 1:800, Affinity Biosciences Cincinnati, OH, USA), rabbit anti-CREB antibody (9197, 1:1000, CST), rabbit anti-p-CREB antibody (9198, 1:800, CST), rabbit anti-BDNF antibody (DF6387, 1:700, Affinity Biosciences Cincinnati, OH, USA), and rabbit anti-GAPDH antibody (5174, 1:1000, CST).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control, Saline

a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of PDE4A, PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.

Journal: Communications Biology

Article Title: Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction

doi: 10.1038/s42003-022-03029-0

Figure Lengend Snippet: a Real-time polymerase chain reaction (RT-PCR) was used to measure mRNA expression of PDE4A, PDE4B, PDE4C, and PDE4D in the aorta tissues of control mice and hypertensive mice (fold change vs. one of controls). b Representative western blot showing PDE4D expression in aorta tissues. c Quantification of PDE4D expression normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein. d Representative immunohistochemical staining of PDE4D. Arrows indicate positive areas. e Quantification of the percentage of PDE4D-positive area. n = 4 in control group, n = 8 in hypertensive group. Data are expressed as mean ± standard error of mean (SEM). Two-tailed Student’s t test was performed to compare differences between two groups. * p < 0.05, *** p < 0.001. L lumen.

Article Snippet: The target protein was probed with numerous antibodies: PDE4A (1:1000, Thermo Fisher, Cat#:PA5-115730), PDE4B (1:1000, Cell Signaling Technology, Cat#: 72096S), PDE4C (1:1000, Thermo Fisher, Cat#: PA5-106624), PDE4D (1:1000, Abcam, Cat#: ab171750), AMP-activated protein kinase (AMPK; 1:1000, Cell Signaling Technology, Cat#: 2532S), Phospho-AMPK (1:1000, Cell Signaling Technology, Cat#: 2535S), myosin phosphatase targeting subunit 1 (MYPT1; 1:1000, Cell Signaling Technology, Cat#: 2634S), Phospho-MYPT1 (1:500, Cell Signaling Technology, Cat#: 5163S), MLC (1:1000, Cell Signaling Technology, Cat#: 8505S), and Phospho-MLC (1:1000, Cell Signaling Technology, Cat#: 3675S), respectively.

Techniques: Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Expressing, Control, Western Blot, Immunohistochemical staining, Staining, Two Tailed Test